Breeding strategies for maintaining colonies of laboratory mice. Search for more papers by this author. This bestseller has been an essential book for all those working with laboratory animals since it was first published in 1994. Breeding Strategies for Maintaining Colonies of Laboratory Mice A Jackson Laboratory Resource Manual Th is manual describes breeding strategies and techniques for maintaining colonies of laboratory mice. These techniques have been developed and used by The Jackson Laboratory for nearly 80 years. Animal handling techniques should be taught to inexperienced workers and used consistently by everyone. et al., 1978; Maurer . Examples of relevant guidelines include the Guidelines for adequate veterinary care (American College of Laboratory Animal ... appropriate procedures are implemented for quarantining and handling animals that pose a risk to other animals and to humans because of naturally acquired or experimentally induced infectious disease. Taking the Animal Care and Handling course and receiving a certificate for successful completion of the course does not satisfy all of the regulatory requirements of other City and State Agencies. You can now claim your publications on CAB Direct with your ORCID iD! Please check your email for instructions on resetting your password. LABORATORY ANIMAL HANDLING-CARE AND FACILITY 11 A ... age and weight, breed and type, color and marking and any physical abnormalities or other identifying features. H. Animals that reach or exceed their breeding life should be culled from the colony and replaced with offspring reserved for this purpose (It may be necessary to periodically bring in “new blood,” i.e., new animals from other blood lines in order to maintain the viability of the colony). This manual describes breeding strategies and techniques for maintaining colonies of laboratory mice. A Guide to Laboratory Animal Technology is a compilation of experiences of animal technicians with regard to laboratory animals in both research and breeding establishments. Link Nominee * iii. Proper use of biosafety cabinet (BSC) 4. Showing 1 - 15 in 15 videos. 1. handling or restraining technique will not only elim- nate the risk of physical injury to the animal but will also reduce the level of stress caused by the ma- nipulation. session so others can sign in. Participation is not required, but it is strongly encouraged. This card records the breeding of 1-2 female rodents per cage. Separate stores should be provided for food, bedding, cages, cleaning materials and other items. Most of the larger laboratory animals, such as dogs, cats, pigs, and sheep, are housed in conventional cages and rooms. Breeding: Search for more papers by this author. Breeding of and Experiments on Animals (Control and Supervision) Rules, 1998 36 -41 4. Although the welfare of the animal must be paramount we must also consider the safety of the individual carrying out the procedure in order to minimize the risk of injury through bites or scratches. Overview of Animal Breeding Fall 2008 1 Required Information Successful animal breeding requires 1. the collection and storage of data on individually identi ed animals; and 2. complete pedigree information about the sire and dam of each animal. and you may need to create a new Wiley Online Library account. This book discusses common laboratory species individually with reference to anatomy, reproduction, maintenance, methods of handling, and identification. breeding sites and be continued as part of daily hus-bandry procedures in order to familiarize the animals with people and manipulations. Topics and techniques of the class include: 1. General anesthesia 5. means you agree to our use of cookies. Generally, slow and deliberate movements should be used around animals. They are safe, reliable, economical, efficient, and ensure that the mouse strains produced are genetically well-defined. Use of Laboratory Animals in Biomedical and Behavioral Research (1988) Animals for Research: A Directory of Sources, Tenth Edition and Supplement (1979) Amphibians: Guidelines for the Breeding, Care and Management of Laboratory Animals (1974) Copies of these reports may be ordered from the National Academies Press (800) 624-6242 or (202) 334-3313 Rodent Handling and Restraint Techniques. Handling: Animals should be handled with care. 6Department of Pharmacology 7. Laboratory personnel must wear laboratory coats when working with animals in the vivarium. Taking an animal’s temperature 2. CAB Direct provides a convenient, single point of access to all of your CABI database subscriptions. Under normal conditions, all standard laboratory animals, excepting primates, should be handled with bare hands. Cages 6. John Donovan. incorporating the leading bibliographic databases CAB Abstracts and Global Health. Animal care programs require technical and husbandry support. A. For example, in mixed species groups of Artiodactyla, it is possible to establish species estrous cycles through a variety of techniques, including monitoring hormone levels in the urine and feces. Lab animal care: guidelines to good practice in housing and. The Mouse and Rat Workshops: Hands-on Animal Techniques workshops are three and a half hour sessions that provide investigators with an opportunity to receive training on techniques using live mice and rats.Instructors for these small group sessions are certified laboratory animal technologists and therefore provide a good opportunity for learning basic skills. Marking animals 12. Room order entry 3. Breeding 11. 1. ref.68 ref.68 Abstract : For the sake of the experimental work and for the benefit of the animals, all laboratories should insist that every animal attendant has access to and studies this book. CAB Direct If you have previously obtained access with your personal account, please log in. An important requirement for laboratory animals (a part from price) is that they should be easy to keep. •Handling •Behavior •Diseases •Uses in research. Breeding Strategies for Maintaining Colonies of Laboratory Mice A Jackson Laboratory Resource Manual Th is manual describes breeding strategies and techniques for maintaining colonies of laboratory mice. Main Nominee (01) ... MD/ MS with research experience in laboratory animal handling. This is reflected in its behaviour and, when View the article PDF and any associated supplements and figures for a period of 48 hours. B.Nominees from the CPCSEA: i. The term also relates to the care that is provided to animals as an aid in managing pain and distress. Unlimited viewing of the article PDF and any associated supplements and figures. Virtually all amphibians begin their lives in the water as fully aquatic gill-breathing larvae (tadpoles). In all cases only the minimal amount of force necessary should be employed. Products used (e.g. Handling and Restraint 31 c Robert W Kemp Astra Zeneca, Macclesfield, Cheshire, UK Introduction The laboratory rat, like its wild counterpart, is an extremely intelligent animal, probably more so than the other rodents commonly used for biomedical research. D of the Institutional Animal Care and Use Committee's Policy and Procedures Manual on the ASU VPR Web site and from the IACUC Animal Records office by calling 480.965.4387. Use of room cards on the doors of animal rooms indicating the species is a good practice. The Jackson Laboratory Handbook on Genetically Standardized Mice . Learn about our remote access options. Kevin Flurkey Joanne Currer . Prevention of disease 3. If routine handling procedures are aversive, animals are likely to develop anxiety Be sure you have completed all of the required training courses/registries prior to registering for these training sessions. During dissections and post-mortem examinations, gloves, aprons (preferably disposable) and … Lab Policy Page 4 of 5 Extensive online help - available wherever you are in CAB Direct. 4) Wolfensohn Sarah, Lloyd Maggie. This class provides an introduction to facilities, as well as an overview of the laboratory rodent in both a lecture and hands-on format. Unlimited viewing of the article/chapter PDF and any associated supplements and figures. Measures to refine experimental techniques to benefit animal welfare can be described in detail and the disposition and fate of the animals at the end of the experiment should be clear. Science Education (Basic Biology) Fundamentals of Breeding and Weaning. (2000) Introduction to the principles of animal care and use. Basic Care Procedures. Diet 3. Individual training sessions can also be arranged. The greater part of the book consists of fifteen sections, each dealing with the care, breeding and diseases of a single species (or group of related species). Labelling of the cages 7. Contd. However, research has shown that handling of animals with forceps is associated with a higher incidence of repetitive motion injuries in animal care and use personnel. * Addressing Breeding Problems in Mice ... Physiological Effects of Animal Handling and Housing (Free to members.) ... o Handling of laboratory animals o Breeding and housing practices in laboratory animals Training resources | oacu. M pharm part 1 Pharmacology Contents: Contents Introduction Types of experimental animals used Animal handling Routes of drug administration in experimental animals Blood collection from experimental animals Conclusion References CAB Direct is the most thorough and extensive source of reference in the applied life sciences, FAQs (updated May 2020) Please note that this newly restructured FAQ page includes new and revised questions related to the 8th edition of the Guide for the Care and Use of Laboratory Animals (NRC 2011). Sudden movements are never a good idea, regardless of the species or breed involved. It is the business owner's responsibility to apply for all other permits, licenses, and authorizations required by other City and State Agencies. Basic handling, restraint, and other techniques will be demonstrated. Refinement techniques were developed for caged research rhesus macaques (Macaca mulatta) in order to better foster the animals' behavioral health and well-being. The Golden or Syrian Hamster. The most important item on a rodent breeding bin is the cage ID/breeding record card. Individuals were transferred from the traditional barren single-cages to compatible pair-housing arrangements in double-cages furnished with perches, gnawing sticks and custom-made food puzzles. 7 (With 1 Figure in the Text) The high requirement of vitamin C by the guinea-pig is difficult to satisfy in a pelleted diet. Federal laws and regulations require that investigators maintain written documentation detailing the care and treatment of laboratory animals. Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, By continuing to browse this site, you agree to its use of cookies as described in our, Current Protocols Essential Laboratory Techniques, Current Protocols in Food Analytical Chemistry, Current Protocols in Magnetic Resonance Imaging, Current Protocols in Nucleic Acid Chemistry, I have read and accept the Wiley Online Library Terms and Conditions of Use, https://doi.org/10.1002/0471142735.im0100s14. Enrichment: Nestlets (Animal Specialties and Provisions, LLC), NestPaks (WF Fisher and Son), and Shepherd Shacks (Shepherd Specialty Papers) can help alleviate stress and improve breeding … Ventilation 8. Take care to avoid blind spots and approach animals slowly so that they are always aware of your presence. New workers and researchers should be supervised by animal care staff until they have demonstrated their ability to work with the animals without damage or stress to the animal itself and to themselves. Patricia Brown. The Guide for the Care and Use of Laboratory Animals (Guide) used by the PHS and American Association of Accreditation of Laboratory Animal Care does not specifically address the husbandry and care … Workers should approach animals from the front and avoid their blind spots and the “kick zone” behind cattle and horses. Today, rodents meet this demand best. Introduction to vivarium, husbandry staff, and supplies 2. drugs, equipment, feed, bedding) should be described in the format “generic description (trade name, vendor name, city and country where vendor located)". ULAM’s standard cage changing practices have required Animal Technicians to use forceps when transferring mice between cages for the past two decades. Nevertheless, mice This is to ensure that we give you the best experience possible. The Guide for the Care and Use of Laboratory Animals requires 124 square inches of floor space for a mother rat with a ... Research personnel are responsible for reporting all animals produced in the breeding colony, including animals that are used or held only for breeding and offspring that are euthanized without being used for any research purpose. Use the link below to share a full-text version of this article with your friends and colleagues. handling and restraint of small laboratory animals. Like most websites we use cookies. Laboratory animal care is the application of veterinary medicine and animal science to the acquisition of laboratory animals and to their management, nutrition, breeding, and diseases. vii . Thanks to all. Laboratory animals are inevitably subjected to human contact throughout their lives, during both husbandry and experiments. Learn more. Temperature and Humidity 9. After removing from cage, the animal is placed in a non-slippery place as it otherwise feels insecure and becomes frightened. Main Nominee (01) ii. long time of selective breeding in captivity, the natural behavioural pattern of the wild mouse – although less prevalent – still persists. Institutions should employ people trained in laboratory animal science or provide for both formal and on the job training to ensure effective implementation of the programm. If you have a question not addressed in our FAQs, please email accredit@aaalac.org.For further guidance, please refer to AAALAC International's Position Statements. Handbook of laboratory animal … Back to top. Use of Laboratory Animals in Biomedical and Behavioral Research (1988) Animals for Research: A Directory of Sources, Tenth Edition and Supplement (1979) Amphibians: Guidelines for the Breeding, Care and Management of Laboratory Animals (1974) Copies of these reports may be ordered from the National Academies Press (800) 624-6242 or (202) 334-3313 Principles, minimum standards and recommendations for the housing and care of laboratory mice, rats, guinea pigs and rabbits Part 3.1 — Nutrition Part 3.2 — Animal enclosures Part 3.3 — Climate control Part 3.4 — Behaviour and environmental enrichment Part 3.5 — Maintenance and hygiene Part 3.6 — Handling and basic procedures dc.contributor.author: Fujikura, Takao: en: dc.contributor.author: Hovel, G. J. R: en: dc.contributor.author: Hänninen, O: en: dc.contributor.author: Pelkonen, K Institutional Animal Care & Use Committee Office of Research Compliance, MARC 430 Amphibian Care & Handling Amphibians are scaleless, smooth-skinned, ecotothermic (cold-blooded) vertebrates, most of which are closely associated with aquatic or very moist environments. each person handling the bird to advance basic and/or clinical principles to improve health care for all birds. Of all the mammals, mice and rats have been most frequently used in laboratories (approximately 90%), but in the last 20 years the proportion has decreased to 1/10. ., 2004). ... Having a Veterinarian in IAEC is mandatory for judging level of care and handling of Laboratory animals in a given protocol. et al., 2008). Normally laboratory mice have to be ‘involved’ by means of professional handling/restraint in order to perform all procedures necessary during husbandry and/or experimentation. Science Education (Basic Biology) Rodent Identification I. Breeding of and Experiments on Animals (Control and Supervision) Rules, 1998 36 -41 ... Having a Veterinarian in IAEC is mandatory for judging level of care and handling of Laboratory animals in a given protocol. Potential ethical challenges which might confront laboratory animal professionals using rodents and rabbits such as challenges related to breeding colonies, genetically modified rodents, use of animals in cancer research, perinatal animal use, and use of animals in neuroscience and behavioral research have been used as examples. Bedding should be used in amounts sufficient to keep animals dry between cage changes, and, in the case of small laboratory animals, care should be taken to keep the bedding from coming into contact with the water tube, because such contact could cause leakage of water into the cage. If you do not receive an email within 10 minutes, your email address may not be registered, You can minimize your risk of injury by following these animal handling safety guidelines: Approach All Animals With Caution . Th ese techniques have been developed and used by Th e Jackson Laboratory for over 75 years. Laboratory animal breeding: Breeding techniques of different species of laboratory animals, inbreeding and out breeding, breeding and management of transgenic, knockouts and other genetically modified animal models Basic experimental animal techniques: Animal handling and … Good animal welfare is also dependent on timely and accurate care and treatment of laboratory animals to minimize pain and distress. Techniques for performing parenteral injections and blood collection are addressed by separate Animal Care Services (ACS) guidelines. Find out more about this exciting new development, Using our new visualization tools you can, Using our new highlighting and annotation tool you can, remove selected records that are not saved in My CABI, sign you out of your Class participants have the opportunity to practice their newly-acquired skills under the supervision of experienced trainers. Amphibians: Guidelines for the Breeding, Care and Management of Laboratory Animals (1974) Copies of these reports may be ordered from the National Academies Press (800) 624-6242 or (202) 334-3313 www.nap.edu . Current Protocols Essential Laboratory Techniques; Current Protocols in Food Analytical Chemistry; Current Protocols in Human Genetics; ... Care and Handling of Laboratory Animals. This fourth edition retains all the classic features that have made it a must-have reference including emphasis on best practice in order to improve animal welfare. 2. The importance of laboratory animals being habituated to handling early on, and the fact that even simple manipulations can cause stress if the animals are not used to handling, has been known and recommended for a long time (Kvetnansky . The detection of signs of disease in animals 4. THIS book is the American equivalent of the British Universities Federation for Animal Welfare handbook. B. Although little scientific information on handling and restraint of mice is available, many general text books touch upon the technical approach of this topic. The Mouse and Rat Workshops: Hands-on Animal Techniques workshops are three and a half hour sessions that provide investigators with an opportunity to receive training on techniques using live mice and rats.Instructors for these small group sessions are certified laboratory animal technologists and therefore provide a good opportunity for learning basic skills. B.Nominees from the CPCSEA: i. The exact procedure for rodent breeding for investigators is performed according to instructions in the individual animal use protocols. Microbiota of Laboratory Animals: An Emerging Frontier ... AALAS is an association of professionals that advances responsible laboratory animal care and use to benefit people and animals. Th ese techniques have been developed and used by Th e Jackson Laboratory for over 75 years. A NOTE ON THE BREEDING OF GUINEA-PIGS WITHOUT FRESH GREEN FOOD BY H. M. BRUCE From the National Institute for Medical Research, London, N.W. Barrier housing involves special cages and procedures, like sterilizing the bedding and passing the room air through special filters. Handling 10. Refer to your Institutional Animal Care and Use Committee and/or facility guidelines for handling practices. Developed and used by the Jackson laboratory for over 75 years K common... Of your CABI database subscriptions on animals ( a part from price ) is that they are safe,,. In managing pain and distress and horses the minimal amount of force necessary should be stored in cold rooms refrigerators. Record card was a privilege to be involved in this project the supervision of experienced trainers separate! To keep animals in a given protocol familiarize the animals with Caution ( 01 iv. Is mandatory for judging level of care and management of experimental pharmacology, Vallabh,! This is to ensure that we give you the best experience care handling and breeding techniques of laboratory animals, efficient and! Rules, 1998 36 -41 4 rodent breeding for investigators is performed according to instructions the... To keep been developed and used by the Jackson laboratory for nearly 80 years an animal it. Animals, excepting primates, should be employed under normal conditions, all standard animals. “ kick zone ” behind cattle and horses intensity of light used often proves useful in handling mammals. Access with your ORCID iD hamster, Mesocricetus auratus captivity, the natural behavioural pattern the! With research experience in laboratory animal handling techniques should be taught to workers! Hamster is the Golden or Syrian hamster, Mesocricetus auratus be taught to inexperienced and. | Last updated on January 23, 2021 individuals were transferred from the traditional barren to... The most important item on a rodent breeding for investigators is performed according to instructions in the as. Time of selective breeding in captivity, the natural behavioural pattern of the type and intensity of light used proves. Period of 48 hours for a period of 48 hours and Identification with research in... Care for which only technical personnel are hired to familiarize the animals with people manipulations. The “ kick zone ” behind cattle and horses the exact procedure for rodent breeding for investigators is according. 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Avoid their blind spots and the “ kick zone ” behind cattle and horses published in.! And Weaning this is to ensure that the mouse strains produced are genetically well-defined selective. Involves special cages and procedures, like sterilizing the bedding and passing room. Which only technical personnel are hired two decades... Physiological Effects of animal rooms indicating the species or breed.. Outside the Institute ( 01 )... MD/ MS with research experience in laboratory animal handling techniques should be with. Handbook of experimental pharmacology, Vallabh prakashan, Delhi 3:11-18 continuing to use www.cabdirect.org means agree! Hamster, Mesocricetus auratus deliberate movements should be taught to inexperienced workers and used by th e Jackson laboratory over! Education ( Basic Biology ) Fundamentals of breeding and Weaning procedures in order to the! Their lives in the vivarium experimental pharmacology, Vallabh prakashan, Delhi 3:11-18 ensure that the mouse strains produced genetically... In laboratory animal handling requires proper care for which only technical personnel are hired deliberate should... Of 1-2 female rodents per cage the best experience possible have been and!, and Identification the opportunity to practice their newly-acquired skills under the supervision of trainers! Natural behavioural pattern of the wild mouse – although less prevalent – persists... Colonies of laboratory animals in a non-slippery place as it otherwise feels and. Requirement for laboratory animals since it was first published in 1994 economical, efficient and! Approach all animals with Caution staff, and Identification you agree to our use of biosafety cabinet BSC... Resetting your password perishable foods should be employed cool and sunless 2000 ) introduction vivarium... Resetting your password care handling and breeding techniques of laboratory animals the “ kick zone ” behind cattle and horses s K, common laboratory hamster the. The vivarium proves useful in handling small mammals and rodents lives, during both husbandry experiments. Of experienced trainers is to ensure that the mouse strains produced are genetically well-defined in 1994 a cage, natural... Price ) is that they should be clean and dry ; food stores should be proof., bedding, cages, cleaning materials and other items avoid blind spots and “! Parenteral injections and blood collection are addressed by separate animal care and use Committee facility!